![]() Use the table below to select the right loading control for your sample type. Similarly specific antibodies to GAPDH are used to measure GAPDH expression as a protein standard in western blotting experiments. This ensures that you will be able to distinguish between the bands. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. Select a loading control that has a different molecular weight to the protein of interest. Loading controls can show if this effect has occurred and allows the variation in binding to be corrected for.Īs an example, to be published in many Nature journals, a paper must meet four general criteria, the first of which is that it must "provide strong evidence for its conclusions." This directly correlates to the necessity of controls to prove that results obtained are valid. This may result in more variation in binding compared to other areas of the gel. Proteins in the outer lanes of the gel are transferred to the membranes in a position close to the frame. This is an issue that is particularly important in signaling assays or experiments where a large number of lanes are being run at once. This is imperative when comparisons are made of the protein expression levels between samples. They can be used to check that there has been even transfer from the gel to the membrane across the whole gel. Loading controls have a second role as a control in western blots. When lanes have not been loaded evenly, loading controls can be used to quantify the protein amounts in each lane by using the density of the loading control band to correct for the differences in loading. Highly specific and rigorously validated in-house, GAPDH (D4C6R) Mouse Monoclonal Antibody (CST 97166) is ready to ship. Here the main reasons you need to be using loading controls: Loading controls can be used to normalize the levels of protein detected by confirming that protein loading is the same across the gel. Why choose our loading control antibodies?.Loading controls are absolutely essential to ensure the reliability of your data when comparing expression of a protein in different samples. Anti-GAPDH Antibody (0411) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry. Housekeeping genes are often chosen for use this purpose. Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample you are studying.
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